Review



image-it fixative solutions #fb002  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher image-it fixative solutions #fb002
    Image It Fixative Solutions #Fb002, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image-it+fixative+solution/4++paraformaldehyde/10__1016_slash_j__cej__2025__164496-178-13-17
    Average 90 stars, based on 1 article reviews
    image-it fixative solutions #fb002 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Article Title: Angiopep-2-Functionalized Lipid Cubosomes for Blood-Brain Barrier Crossing and Glioblastoma Treatment.
    Article Snippet: Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis.. Nanoparticles offer a way to cross the blood−brain barrier (BBB) and deliver precise therapies to tumor sites with reduced side effects.. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded with cisplatin (CDDP) and temozolomide (TMZ) for crossing the BBB and providing targeted glioblastoma therapy.

    Article Title: A Versatile Intestine‐on‐Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease
    Article Snippet: The IEB model was fixed with Image‐iT fixative solution (Invitrogen, Thermo Fisher Scientific) for 15 min at RT.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-DDX4 antibody (1:200) Abcam Cat# ab27591; RRID: AB_11139638 Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:500) Thermo Fisher Scientific Cat# A-11001; RRID: AB_2534069 (Continued on next page) 2 STAR Protocols 6, 103661, March 21, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Cell Signaling Technology Cat# 2112 BlockAid blocking solution Thermo Fisher Scientific Cat# B10710 Image-iT fixative solution Thermo Fisher Scientific Cat# R37814 Triton X-100 Sigma Cat# 93443 Phosphate-buffered saline (PBS, pH 7.4) Gibco Cat# 10010023 Minimum essential medium a (MEMa) Gibco Cat# 12561056 Glial cell line-derived neurotrophic factor (GDNF) PeproTech Cat# 450-10 Basic fibroblast growth factor (bFGF) PeproTech Cat# 100-18B Insulin Sigma-Aldrich Cat# I 2643 Bovine serum albumin(BSA) MP Biomedicals Cat# 810661 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipoprotein Sigma-Aldrich Cat# L4646 GlutaMAX Gibco Cat# 35050061 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Gibco Cat# 15630-080 Non-essential amino acids (NEAA) Gibco Cat# 11140-050 Sodium selenite Sigma Cat# S5261 Putrescine Sigma Cat# P5780 2-methoxyethanol (2-ME) Sigma Cat# M3145 Transferrin Sigma Cat# T0665 Ascorbic acid Sigma Cat# M4544 Critical commercial assays Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit Thermo Fisher Scientific Cat# C10457 Experimental models: Organisms/strains Mouse; C57BL/6J, 4-days-old, male mice Speford Biotechnology Company (Beijing, China) N/A Software and algorithms ZEN lite version 2 Zeiss https://www.zeiss.com/microscopy/zh/ products/software.html ImageJ v.1.8.0 ImageJ software https://imagej.net/ij/ GraphPad Prism v.8 GraphPad Software https://www.graphpad.com/ Other 96-well culture plates Costar 3599 Confocal microscope Zeiss LSM 780 Rocking lab shaker Bolaiyan BLY-100E Adhesion microscope slides Citotest 188105 Microscope cover glasses BRAND GMBH + CO KG 470820 ll OPEN ACCESSProtocol

    Article Title: Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8 + T cell responses
    Article Snippet: Isolated CD8 + cells were then incubated for 20 min with the indicated MMrs (10 nM) diluted in 2% goat serum in PBS and fixed with 4% Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 min. CD8 + cells were then cytospun on a microscope slide and blocked with 10% goat serum and Human TruStain FcX (RRID:AB_2818986; 1:20) for 20 min at 4°C.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications.
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: G3BP1::CSF1R: a new and actionable gene fusion in acute megakaryoblastic leukemia
    Article Snippet: For analysis of pERK1/2 and pSTAT5, cells were fixed in Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 minutes at room temperature, permeabilized in 90% methanal for 30 minutes on ice and stained with the antibodies for 1 hour at room temperature.

    Blocking Assay:

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Article Title: Angiopep-2-Functionalized Lipid Cubosomes for Blood-Brain Barrier Crossing and Glioblastoma Treatment.
    Article Snippet: Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis.. Nanoparticles offer a way to cross the blood−brain barrier (BBB) and deliver precise therapies to tumor sites with reduced side effects.. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded with cisplatin (CDDP) and temozolomide (TMZ) for crossing the BBB and providing targeted glioblastoma therapy.

    Article Title: A Versatile Intestine‐on‐Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease
    Article Snippet: The IEB model was fixed with Image‐iT fixative solution (Invitrogen, Thermo Fisher Scientific) for 15 min at RT.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-DDX4 antibody (1:200) Abcam Cat# ab27591; RRID: AB_11139638 Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:500) Thermo Fisher Scientific Cat# A-11001; RRID: AB_2534069 (Continued on next page) 2 STAR Protocols 6, 103661, March 21, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Cell Signaling Technology Cat# 2112 BlockAid blocking solution Thermo Fisher Scientific Cat# B10710 Image-iT fixative solution Thermo Fisher Scientific Cat# R37814 Triton X-100 Sigma Cat# 93443 Phosphate-buffered saline (PBS, pH 7.4) Gibco Cat# 10010023 Minimum essential medium a (MEMa) Gibco Cat# 12561056 Glial cell line-derived neurotrophic factor (GDNF) PeproTech Cat# 450-10 Basic fibroblast growth factor (bFGF) PeproTech Cat# 100-18B Insulin Sigma-Aldrich Cat# I 2643 Bovine serum albumin(BSA) MP Biomedicals Cat# 810661 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipoprotein Sigma-Aldrich Cat# L4646 GlutaMAX Gibco Cat# 35050061 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Gibco Cat# 15630-080 Non-essential amino acids (NEAA) Gibco Cat# 11140-050 Sodium selenite Sigma Cat# S5261 Putrescine Sigma Cat# P5780 2-methoxyethanol (2-ME) Sigma Cat# M3145 Transferrin Sigma Cat# T0665 Ascorbic acid Sigma Cat# M4544 Critical commercial assays Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit Thermo Fisher Scientific Cat# C10457 Experimental models: Organisms/strains Mouse; C57BL/6J, 4-days-old, male mice Speford Biotechnology Company (Beijing, China) N/A Software and algorithms ZEN lite version 2 Zeiss https://www.zeiss.com/microscopy/zh/ products/software.html ImageJ v.1.8.0 ImageJ software https://imagej.net/ij/ GraphPad Prism v.8 GraphPad Software https://www.graphpad.com/ Other 96-well culture plates Costar 3599 Confocal microscope Zeiss LSM 780 Rocking lab shaker Bolaiyan BLY-100E Adhesion microscope slides Citotest 188105 Microscope cover glasses BRAND GMBH + CO KG 470820 ll OPEN ACCESSProtocol

    Article Title: Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8 + T cell responses
    Article Snippet: Isolated CD8 + cells were then incubated for 20 min with the indicated MMrs (10 nM) diluted in 2% goat serum in PBS and fixed with 4% Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 min. CD8 + cells were then cytospun on a microscope slide and blocked with 10% goat serum and Human TruStain FcX (RRID:AB_2818986; 1:20) for 20 min at 4°C.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications.
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: G3BP1::CSF1R: a new and actionable gene fusion in acute megakaryoblastic leukemia
    Article Snippet: For analysis of pERK1/2 and pSTAT5, cells were fixed in Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 minutes at room temperature, permeabilized in 90% methanal for 30 minutes on ice and stained with the antibodies for 1 hour at room temperature.

    Saline:

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Article Title: Angiopep-2-Functionalized Lipid Cubosomes for Blood-Brain Barrier Crossing and Glioblastoma Treatment.
    Article Snippet: Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis.. Nanoparticles offer a way to cross the blood−brain barrier (BBB) and deliver precise therapies to tumor sites with reduced side effects.. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded with cisplatin (CDDP) and temozolomide (TMZ) for crossing the BBB and providing targeted glioblastoma therapy.

    Article Title: A Versatile Intestine‐on‐Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease
    Article Snippet: The IEB model was fixed with Image‐iT fixative solution (Invitrogen, Thermo Fisher Scientific) for 15 min at RT.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-DDX4 antibody (1:200) Abcam Cat# ab27591; RRID: AB_11139638 Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:500) Thermo Fisher Scientific Cat# A-11001; RRID: AB_2534069 (Continued on next page) 2 STAR Protocols 6, 103661, March 21, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Cell Signaling Technology Cat# 2112 BlockAid blocking solution Thermo Fisher Scientific Cat# B10710 Image-iT fixative solution Thermo Fisher Scientific Cat# R37814 Triton X-100 Sigma Cat# 93443 Phosphate-buffered saline (PBS, pH 7.4) Gibco Cat# 10010023 Minimum essential medium a (MEMa) Gibco Cat# 12561056 Glial cell line-derived neurotrophic factor (GDNF) PeproTech Cat# 450-10 Basic fibroblast growth factor (bFGF) PeproTech Cat# 100-18B Insulin Sigma-Aldrich Cat# I 2643 Bovine serum albumin(BSA) MP Biomedicals Cat# 810661 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipoprotein Sigma-Aldrich Cat# L4646 GlutaMAX Gibco Cat# 35050061 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Gibco Cat# 15630-080 Non-essential amino acids (NEAA) Gibco Cat# 11140-050 Sodium selenite Sigma Cat# S5261 Putrescine Sigma Cat# P5780 2-methoxyethanol (2-ME) Sigma Cat# M3145 Transferrin Sigma Cat# T0665 Ascorbic acid Sigma Cat# M4544 Critical commercial assays Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit Thermo Fisher Scientific Cat# C10457 Experimental models: Organisms/strains Mouse; C57BL/6J, 4-days-old, male mice Speford Biotechnology Company (Beijing, China) N/A Software and algorithms ZEN lite version 2 Zeiss https://www.zeiss.com/microscopy/zh/ products/software.html ImageJ v.1.8.0 ImageJ software https://imagej.net/ij/ GraphPad Prism v.8 GraphPad Software https://www.graphpad.com/ Other 96-well culture plates Costar 3599 Confocal microscope Zeiss LSM 780 Rocking lab shaker Bolaiyan BLY-100E Adhesion microscope slides Citotest 188105 Microscope cover glasses BRAND GMBH + CO KG 470820 ll OPEN ACCESSProtocol

    Article Title: Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8 + T cell responses
    Article Snippet: Isolated CD8 + cells were then incubated for 20 min with the indicated MMrs (10 nM) diluted in 2% goat serum in PBS and fixed with 4% Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 min. CD8 + cells were then cytospun on a microscope slide and blocked with 10% goat serum and Human TruStain FcX (RRID:AB_2818986; 1:20) for 20 min at 4°C.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications.
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: G3BP1::CSF1R: a new and actionable gene fusion in acute megakaryoblastic leukemia
    Article Snippet: For analysis of pERK1/2 and pSTAT5, cells were fixed in Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 minutes at room temperature, permeabilized in 90% methanal for 30 minutes on ice and stained with the antibodies for 1 hour at room temperature.

    Software:

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Article Title: Angiopep-2-Functionalized Lipid Cubosomes for Blood-Brain Barrier Crossing and Glioblastoma Treatment.
    Article Snippet: Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis.. Nanoparticles offer a way to cross the blood−brain barrier (BBB) and deliver precise therapies to tumor sites with reduced side effects.. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded with cisplatin (CDDP) and temozolomide (TMZ) for crossing the BBB and providing targeted glioblastoma therapy.

    Article Title: A Versatile Intestine‐on‐Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease
    Article Snippet: The IEB model was fixed with Image‐iT fixative solution (Invitrogen, Thermo Fisher Scientific) for 15 min at RT.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-DDX4 antibody (1:200) Abcam Cat# ab27591; RRID: AB_11139638 Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:500) Thermo Fisher Scientific Cat# A-11001; RRID: AB_2534069 (Continued on next page) 2 STAR Protocols 6, 103661, March 21, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Cell Signaling Technology Cat# 2112 BlockAid blocking solution Thermo Fisher Scientific Cat# B10710 Image-iT fixative solution Thermo Fisher Scientific Cat# R37814 Triton X-100 Sigma Cat# 93443 Phosphate-buffered saline (PBS, pH 7.4) Gibco Cat# 10010023 Minimum essential medium a (MEMa) Gibco Cat# 12561056 Glial cell line-derived neurotrophic factor (GDNF) PeproTech Cat# 450-10 Basic fibroblast growth factor (bFGF) PeproTech Cat# 100-18B Insulin Sigma-Aldrich Cat# I 2643 Bovine serum albumin(BSA) MP Biomedicals Cat# 810661 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipoprotein Sigma-Aldrich Cat# L4646 GlutaMAX Gibco Cat# 35050061 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Gibco Cat# 15630-080 Non-essential amino acids (NEAA) Gibco Cat# 11140-050 Sodium selenite Sigma Cat# S5261 Putrescine Sigma Cat# P5780 2-methoxyethanol (2-ME) Sigma Cat# M3145 Transferrin Sigma Cat# T0665 Ascorbic acid Sigma Cat# M4544 Critical commercial assays Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit Thermo Fisher Scientific Cat# C10457 Experimental models: Organisms/strains Mouse; C57BL/6J, 4-days-old, male mice Speford Biotechnology Company (Beijing, China) N/A Software and algorithms ZEN lite version 2 Zeiss https://www.zeiss.com/microscopy/zh/ products/software.html ImageJ v.1.8.0 ImageJ software https://imagej.net/ij/ GraphPad Prism v.8 GraphPad Software https://www.graphpad.com/ Other 96-well culture plates Costar 3599 Confocal microscope Zeiss LSM 780 Rocking lab shaker Bolaiyan BLY-100E Adhesion microscope slides Citotest 188105 Microscope cover glasses BRAND GMBH + CO KG 470820 ll OPEN ACCESSProtocol

    Article Title: Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8 + T cell responses
    Article Snippet: Isolated CD8 + cells were then incubated for 20 min with the indicated MMrs (10 nM) diluted in 2% goat serum in PBS and fixed with 4% Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 min. CD8 + cells were then cytospun on a microscope slide and blocked with 10% goat serum and Human TruStain FcX (RRID:AB_2818986; 1:20) for 20 min at 4°C.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications.
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: G3BP1::CSF1R: a new and actionable gene fusion in acute megakaryoblastic leukemia
    Article Snippet: For analysis of pERK1/2 and pSTAT5, cells were fixed in Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 minutes at room temperature, permeabilized in 90% methanal for 30 minutes on ice and stained with the antibodies for 1 hour at room temperature.

    Microscopy:

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Article Title: Angiopep-2-Functionalized Lipid Cubosomes for Blood-Brain Barrier Crossing and Glioblastoma Treatment.
    Article Snippet: Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis.. Nanoparticles offer a way to cross the blood−brain barrier (BBB) and deliver precise therapies to tumor sites with reduced side effects.. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded with cisplatin (CDDP) and temozolomide (TMZ) for crossing the BBB and providing targeted glioblastoma therapy.

    Article Title: A Versatile Intestine‐on‐Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease
    Article Snippet: The IEB model was fixed with Image‐iT fixative solution (Invitrogen, Thermo Fisher Scientific) for 15 min at RT.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-DDX4 antibody (1:200) Abcam Cat# ab27591; RRID: AB_11139638 Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:500) Thermo Fisher Scientific Cat# A-11001; RRID: AB_2534069 (Continued on next page) 2 STAR Protocols 6, 103661, March 21, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Cell Signaling Technology Cat# 2112 BlockAid blocking solution Thermo Fisher Scientific Cat# B10710 Image-iT fixative solution Thermo Fisher Scientific Cat# R37814 Triton X-100 Sigma Cat# 93443 Phosphate-buffered saline (PBS, pH 7.4) Gibco Cat# 10010023 Minimum essential medium a (MEMa) Gibco Cat# 12561056 Glial cell line-derived neurotrophic factor (GDNF) PeproTech Cat# 450-10 Basic fibroblast growth factor (bFGF) PeproTech Cat# 100-18B Insulin Sigma-Aldrich Cat# I 2643 Bovine serum albumin(BSA) MP Biomedicals Cat# 810661 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipoprotein Sigma-Aldrich Cat# L4646 GlutaMAX Gibco Cat# 35050061 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Gibco Cat# 15630-080 Non-essential amino acids (NEAA) Gibco Cat# 11140-050 Sodium selenite Sigma Cat# S5261 Putrescine Sigma Cat# P5780 2-methoxyethanol (2-ME) Sigma Cat# M3145 Transferrin Sigma Cat# T0665 Ascorbic acid Sigma Cat# M4544 Critical commercial assays Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit Thermo Fisher Scientific Cat# C10457 Experimental models: Organisms/strains Mouse; C57BL/6J, 4-days-old, male mice Speford Biotechnology Company (Beijing, China) N/A Software and algorithms ZEN lite version 2 Zeiss https://www.zeiss.com/microscopy/zh/ products/software.html ImageJ v.1.8.0 ImageJ software https://imagej.net/ij/ GraphPad Prism v.8 GraphPad Software https://www.graphpad.com/ Other 96-well culture plates Costar 3599 Confocal microscope Zeiss LSM 780 Rocking lab shaker Bolaiyan BLY-100E Adhesion microscope slides Citotest 188105 Microscope cover glasses BRAND GMBH + CO KG 470820 ll OPEN ACCESSProtocol

    Article Title: Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8 + T cell responses
    Article Snippet: Isolated CD8 + cells were then incubated for 20 min with the indicated MMrs (10 nM) diluted in 2% goat serum in PBS and fixed with 4% Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 min. CD8 + cells were then cytospun on a microscope slide and blocked with 10% goat serum and Human TruStain FcX (RRID:AB_2818986; 1:20) for 20 min at 4°C.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications.
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: G3BP1::CSF1R: a new and actionable gene fusion in acute megakaryoblastic leukemia
    Article Snippet: For analysis of pERK1/2 and pSTAT5, cells were fixed in Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 minutes at room temperature, permeabilized in 90% methanal for 30 minutes on ice and stained with the antibodies for 1 hour at room temperature.

    Cell Culture:

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Article Title: Angiopep-2-Functionalized Lipid Cubosomes for Blood-Brain Barrier Crossing and Glioblastoma Treatment.
    Article Snippet: Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis.. Nanoparticles offer a way to cross the blood−brain barrier (BBB) and deliver precise therapies to tumor sites with reduced side effects.. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded with cisplatin (CDDP) and temozolomide (TMZ) for crossing the BBB and providing targeted glioblastoma therapy.

    Article Title: A Versatile Intestine‐on‐Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease
    Article Snippet: The IEB model was fixed with Image‐iT fixative solution (Invitrogen, Thermo Fisher Scientific) for 15 min at RT.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-DDX4 antibody (1:200) Abcam Cat# ab27591; RRID: AB_11139638 Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:500) Thermo Fisher Scientific Cat# A-11001; RRID: AB_2534069 (Continued on next page) 2 STAR Protocols 6, 103661, March 21, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Cell Signaling Technology Cat# 2112 BlockAid blocking solution Thermo Fisher Scientific Cat# B10710 Image-iT fixative solution Thermo Fisher Scientific Cat# R37814 Triton X-100 Sigma Cat# 93443 Phosphate-buffered saline (PBS, pH 7.4) Gibco Cat# 10010023 Minimum essential medium a (MEMa) Gibco Cat# 12561056 Glial cell line-derived neurotrophic factor (GDNF) PeproTech Cat# 450-10 Basic fibroblast growth factor (bFGF) PeproTech Cat# 100-18B Insulin Sigma-Aldrich Cat# I 2643 Bovine serum albumin(BSA) MP Biomedicals Cat# 810661 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipoprotein Sigma-Aldrich Cat# L4646 GlutaMAX Gibco Cat# 35050061 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Gibco Cat# 15630-080 Non-essential amino acids (NEAA) Gibco Cat# 11140-050 Sodium selenite Sigma Cat# S5261 Putrescine Sigma Cat# P5780 2-methoxyethanol (2-ME) Sigma Cat# M3145 Transferrin Sigma Cat# T0665 Ascorbic acid Sigma Cat# M4544 Critical commercial assays Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit Thermo Fisher Scientific Cat# C10457 Experimental models: Organisms/strains Mouse; C57BL/6J, 4-days-old, male mice Speford Biotechnology Company (Beijing, China) N/A Software and algorithms ZEN lite version 2 Zeiss https://www.zeiss.com/microscopy/zh/ products/software.html ImageJ v.1.8.0 ImageJ software https://imagej.net/ij/ GraphPad Prism v.8 GraphPad Software https://www.graphpad.com/ Other 96-well culture plates Costar 3599 Confocal microscope Zeiss LSM 780 Rocking lab shaker Bolaiyan BLY-100E Adhesion microscope slides Citotest 188105 Microscope cover glasses BRAND GMBH + CO KG 470820 ll OPEN ACCESSProtocol

    Article Title: Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8 + T cell responses
    Article Snippet: Isolated CD8 + cells were then incubated for 20 min with the indicated MMrs (10 nM) diluted in 2% goat serum in PBS and fixed with 4% Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 min. CD8 + cells were then cytospun on a microscope slide and blocked with 10% goat serum and Human TruStain FcX (RRID:AB_2818986; 1:20) for 20 min at 4°C.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications.
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: G3BP1::CSF1R: a new and actionable gene fusion in acute megakaryoblastic leukemia
    Article Snippet: For analysis of pERK1/2 and pSTAT5, cells were fixed in Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 minutes at room temperature, permeabilized in 90% methanal for 30 minutes on ice and stained with the antibodies for 1 hour at room temperature.

    Suspension:

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Article Title: Angiopep-2-Functionalized Lipid Cubosomes for Blood-Brain Barrier Crossing and Glioblastoma Treatment.
    Article Snippet: Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis.. Nanoparticles offer a way to cross the blood−brain barrier (BBB) and deliver precise therapies to tumor sites with reduced side effects.. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded with cisplatin (CDDP) and temozolomide (TMZ) for crossing the BBB and providing targeted glioblastoma therapy.

    Article Title: A Versatile Intestine‐on‐Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease
    Article Snippet: The IEB model was fixed with Image‐iT fixative solution (Invitrogen, Thermo Fisher Scientific) for 15 min at RT.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-DDX4 antibody (1:200) Abcam Cat# ab27591; RRID: AB_11139638 Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:500) Thermo Fisher Scientific Cat# A-11001; RRID: AB_2534069 (Continued on next page) 2 STAR Protocols 6, 103661, March 21, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Cell Signaling Technology Cat# 2112 BlockAid blocking solution Thermo Fisher Scientific Cat# B10710 Image-iT fixative solution Thermo Fisher Scientific Cat# R37814 Triton X-100 Sigma Cat# 93443 Phosphate-buffered saline (PBS, pH 7.4) Gibco Cat# 10010023 Minimum essential medium a (MEMa) Gibco Cat# 12561056 Glial cell line-derived neurotrophic factor (GDNF) PeproTech Cat# 450-10 Basic fibroblast growth factor (bFGF) PeproTech Cat# 100-18B Insulin Sigma-Aldrich Cat# I 2643 Bovine serum albumin(BSA) MP Biomedicals Cat# 810661 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipoprotein Sigma-Aldrich Cat# L4646 GlutaMAX Gibco Cat# 35050061 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Gibco Cat# 15630-080 Non-essential amino acids (NEAA) Gibco Cat# 11140-050 Sodium selenite Sigma Cat# S5261 Putrescine Sigma Cat# P5780 2-methoxyethanol (2-ME) Sigma Cat# M3145 Transferrin Sigma Cat# T0665 Ascorbic acid Sigma Cat# M4544 Critical commercial assays Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit Thermo Fisher Scientific Cat# C10457 Experimental models: Organisms/strains Mouse; C57BL/6J, 4-days-old, male mice Speford Biotechnology Company (Beijing, China) N/A Software and algorithms ZEN lite version 2 Zeiss https://www.zeiss.com/microscopy/zh/ products/software.html ImageJ v.1.8.0 ImageJ software https://imagej.net/ij/ GraphPad Prism v.8 GraphPad Software https://www.graphpad.com/ Other 96-well culture plates Costar 3599 Confocal microscope Zeiss LSM 780 Rocking lab shaker Bolaiyan BLY-100E Adhesion microscope slides Citotest 188105 Microscope cover glasses BRAND GMBH + CO KG 470820 ll OPEN ACCESSProtocol

    Article Title: Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8 + T cell responses
    Article Snippet: Isolated CD8 + cells were then incubated for 20 min with the indicated MMrs (10 nM) diluted in 2% goat serum in PBS and fixed with 4% Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 min. CD8 + cells were then cytospun on a microscope slide and blocked with 10% goat serum and Human TruStain FcX (RRID:AB_2818986; 1:20) for 20 min at 4°C.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications.
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: G3BP1::CSF1R: a new and actionable gene fusion in acute megakaryoblastic leukemia
    Article Snippet: For analysis of pERK1/2 and pSTAT5, cells were fixed in Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 minutes at room temperature, permeabilized in 90% methanal for 30 minutes on ice and stained with the antibodies for 1 hour at room temperature.

    Isolation:

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Article Title: Angiopep-2-Functionalized Lipid Cubosomes for Blood-Brain Barrier Crossing and Glioblastoma Treatment.
    Article Snippet: Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis.. Nanoparticles offer a way to cross the blood−brain barrier (BBB) and deliver precise therapies to tumor sites with reduced side effects.. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded with cisplatin (CDDP) and temozolomide (TMZ) for crossing the BBB and providing targeted glioblastoma therapy.

    Article Title: A Versatile Intestine‐on‐Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease
    Article Snippet: The IEB model was fixed with Image‐iT fixative solution (Invitrogen, Thermo Fisher Scientific) for 15 min at RT.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-DDX4 antibody (1:200) Abcam Cat# ab27591; RRID: AB_11139638 Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:500) Thermo Fisher Scientific Cat# A-11001; RRID: AB_2534069 (Continued on next page) 2 STAR Protocols 6, 103661, March 21, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Cell Signaling Technology Cat# 2112 BlockAid blocking solution Thermo Fisher Scientific Cat# B10710 Image-iT fixative solution Thermo Fisher Scientific Cat# R37814 Triton X-100 Sigma Cat# 93443 Phosphate-buffered saline (PBS, pH 7.4) Gibco Cat# 10010023 Minimum essential medium a (MEMa) Gibco Cat# 12561056 Glial cell line-derived neurotrophic factor (GDNF) PeproTech Cat# 450-10 Basic fibroblast growth factor (bFGF) PeproTech Cat# 100-18B Insulin Sigma-Aldrich Cat# I 2643 Bovine serum albumin(BSA) MP Biomedicals Cat# 810661 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipoprotein Sigma-Aldrich Cat# L4646 GlutaMAX Gibco Cat# 35050061 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Gibco Cat# 15630-080 Non-essential amino acids (NEAA) Gibco Cat# 11140-050 Sodium selenite Sigma Cat# S5261 Putrescine Sigma Cat# P5780 2-methoxyethanol (2-ME) Sigma Cat# M3145 Transferrin Sigma Cat# T0665 Ascorbic acid Sigma Cat# M4544 Critical commercial assays Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit Thermo Fisher Scientific Cat# C10457 Experimental models: Organisms/strains Mouse; C57BL/6J, 4-days-old, male mice Speford Biotechnology Company (Beijing, China) N/A Software and algorithms ZEN lite version 2 Zeiss https://www.zeiss.com/microscopy/zh/ products/software.html ImageJ v.1.8.0 ImageJ software https://imagej.net/ij/ GraphPad Prism v.8 GraphPad Software https://www.graphpad.com/ Other 96-well culture plates Costar 3599 Confocal microscope Zeiss LSM 780 Rocking lab shaker Bolaiyan BLY-100E Adhesion microscope slides Citotest 188105 Microscope cover glasses BRAND GMBH + CO KG 470820 ll OPEN ACCESSProtocol

    Article Title: Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8 + T cell responses
    Article Snippet: Isolated CD8 + cells were then incubated for 20 min with the indicated MMrs (10 nM) diluted in 2% goat serum in PBS and fixed with 4% Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 min. CD8 + cells were then cytospun on a microscope slide and blocked with 10% goat serum and Human TruStain FcX (RRID:AB_2818986; 1:20) for 20 min at 4°C.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications.
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: G3BP1::CSF1R: a new and actionable gene fusion in acute megakaryoblastic leukemia
    Article Snippet: For analysis of pERK1/2 and pSTAT5, cells were fixed in Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 minutes at room temperature, permeabilized in 90% methanal for 30 minutes on ice and stained with the antibodies for 1 hour at room temperature.

    Incubation:

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen
    Article Snippet: Image-iT fixative solution , Thermo Fisher Scientific , Cat# R37814.

    Article Title: Angiopep-2-Functionalized Lipid Cubosomes for Blood-Brain Barrier Crossing and Glioblastoma Treatment.
    Article Snippet: Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis.. Nanoparticles offer a way to cross the blood−brain barrier (BBB) and deliver precise therapies to tumor sites with reduced side effects.. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded with cisplatin (CDDP) and temozolomide (TMZ) for crossing the BBB and providing targeted glioblastoma therapy.

    Article Title: A Versatile Intestine‐on‐Chip System for Deciphering the Immunopathogenesis of Inflammatory Bowel Disease
    Article Snippet: The IEB model was fixed with Image‐iT fixative solution (Invitrogen, Thermo Fisher Scientific) for 15 min at RT.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: Protocol for identifying protein synthesis activity in specific cell types of the testicular lumen.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-DDX4 antibody (1:200) Abcam Cat# ab27591; RRID: AB_11139638 Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:500) Thermo Fisher Scientific Cat# A-11001; RRID: AB_2534069 (Continued on next page) 2 STAR Protocols 6, 103661, March 21, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Cycloheximide (CHX) Cell Signaling Technology Cat# 2112 BlockAid blocking solution Thermo Fisher Scientific Cat# B10710 Image-iT fixative solution Thermo Fisher Scientific Cat# R37814 Triton X-100 Sigma Cat# 93443 Phosphate-buffered saline (PBS, pH 7.4) Gibco Cat# 10010023 Minimum essential medium a (MEMa) Gibco Cat# 12561056 Glial cell line-derived neurotrophic factor (GDNF) PeproTech Cat# 450-10 Basic fibroblast growth factor (bFGF) PeproTech Cat# 100-18B Insulin Sigma-Aldrich Cat# I 2643 Bovine serum albumin(BSA) MP Biomedicals Cat# 810661 Fetal bovine serum (FBS) HyClone Cat# SH30396.03 Lipoprotein Sigma-Aldrich Cat# L4646 GlutaMAX Gibco Cat# 35050061 N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) Gibco Cat# 15630-080 Non-essential amino acids (NEAA) Gibco Cat# 11140-050 Sodium selenite Sigma Cat# S5261 Putrescine Sigma Cat# P5780 2-methoxyethanol (2-ME) Sigma Cat# M3145 Transferrin Sigma Cat# T0665 Ascorbic acid Sigma Cat# M4544 Critical commercial assays Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit Thermo Fisher Scientific Cat# C10457 Experimental models: Organisms/strains Mouse; C57BL/6J, 4-days-old, male mice Speford Biotechnology Company (Beijing, China) N/A Software and algorithms ZEN lite version 2 Zeiss https://www.zeiss.com/microscopy/zh/ products/software.html ImageJ v.1.8.0 ImageJ software https://imagej.net/ij/ GraphPad Prism v.8 GraphPad Software https://www.graphpad.com/ Other 96-well culture plates Costar 3599 Confocal microscope Zeiss LSM 780 Rocking lab shaker Bolaiyan BLY-100E Adhesion microscope slides Citotest 188105 Microscope cover glasses BRAND GMBH + CO KG 470820 ll OPEN ACCESSProtocol

    Article Title: Coxsackievirus infection induces direct pancreatic β cell killing but poor antiviral CD8 + T cell responses
    Article Snippet: Isolated CD8 + cells were then incubated for 20 min with the indicated MMrs (10 nM) diluted in 2% goat serum in PBS and fixed with 4% Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 min. CD8 + cells were then cytospun on a microscope slide and blocked with 10% goat serum and Human TruStain FcX (RRID:AB_2818986; 1:20) for 20 min at 4°C.

    Article Title: Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indications.
    Article Snippet: Tumoroids from matched lines were cultured in suspension using OncoPro medium, collected, washed in cold DPBS (-/-), and fixed for 1 h at room temperature in Invitrogen Image-iT Fixative Solution (Thermo Fisher Scientific, cat# FB002).

    Article Title: G3BP1::CSF1R: a new and actionable gene fusion in acute megakaryoblastic leukemia
    Article Snippet: For analysis of pERK1/2 and pSTAT5, cells were fixed in Image-iT Fixative Solution (Thermo Fisher Scientific) for 15 minutes at room temperature, permeabilized in 90% methanal for 30 minutes on ice and stained with the antibodies for 1 hour at room temperature.



    Similar Products

    90
    Thermo Fisher image-it fixative solutions #fb002
    Image It Fixative Solutions #Fb002, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image-it+fixative+solution/4++paraformaldehyde/10__1016_slash_j__cej__2025__164496-178-13-17
    Average 90 stars, based on 1 article reviews
    image-it fixative solutions #fb002 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    97
    Thermo Fisher image i fixative solutions
    Image I Fixative Solutions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image-it+fixative+solution/Paraformaldehyde+fixative+solu/pmc12587183-116-14-17
    Average 97 stars, based on 1 article reviews
    image i fixative solutions - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    99
    Thermo Fisher image it fixative solution
    Image It Fixative Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image-it+fixative+solution/Phosphate/pmc12383883-48-13-25
    Average 99 stars, based on 1 article reviews
    image it fixative solution - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher image-it 4% formaldehyde fixative solution r37814
    Image It 4% Formaldehyde Fixative Solution R37814, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image-it+fixative+solution/image+ittm+fixative+solution/pm40317818-239-6-11
    Average 90 stars, based on 1 article reviews
    image-it 4% formaldehyde fixative solution r37814 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher image-ittm fixative solutions fb002
    Image Ittm Fixative Solutions Fb002, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image-it+fixative+solution/4++paraformaldehyde/pm40094473__nl4c04867_si_001-16-9-13
    Average 90 stars, based on 1 article reviews
    image-ittm fixative solutions fb002 - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher image-it fixative solution
    Image It Fixative Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image-it+fixative+solution/blockaidtm+blocking+solution/pm40022734-48-71-75
    Average 90 stars, based on 1 article reviews
    image-it fixative solution - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher image-ittm fixative solution
    Image Ittm Fixative Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image-it+fixative+solution/image+ittm+fixative+solution/pmc11845618-84-7-17
    Average 90 stars, based on 1 article reviews
    image-ittm fixative solution - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results